The dyes we used were for the most part, just like the reference dyes. We didn't have an orange colored dye (yellow 5), but instead we got a green dye from a green skittle. For the green skittle dye, it separated into two colors, blue and yellow. The yellow band from the green dye went the same distance as the yellow reference band and the blue band from the green dye went the same distance as the blue reference dye.
Citrus Red 2 might travel similarly to the yellow reference dye, because of its size. The Fast Green FCF might travel similarly to the orange reference dye. Betanin might migrate similarly to the red dye, and Carminic acid might travel similarly to the blue reference dye.
Dog manufacturers might put artificial food colors in their dog food because they want it to look more appealing to dog owners. It gives the food a more tasty appearance, making the owners want to purchase the food for their dog.
People might prefer artificial food coloring over natural dye because it makes the product look better and more appealing. The more vibrant and more significant the colors are, the more you would most likely want to have it.
Two factors that controlled the distance that the colored dyes traveled is the size of the DNA fragments and the agarose gel that it's in. The force that helps move the dye through the gel is the electrical current. The DNA tends to move away from the negative side and to the positively charged side.
The gel causes the molecules to separate by size because it limits how far the fragments can travel through the gel. In the gel, there are little roadblocks that the fragments have to move around. The smaller the fragment, the faster and easier it can travel toward the positively charged side of the gel.
With molecules of 600, 1000, 2000, and 5000 daltons, I would expect the molecule with 600 daltons to move the furthest, because of its smallest size. The molecule with 5000 daltons would end up closest to the well where you put the molecules in the gel.
Citrus Red 2 might travel similarly to the yellow reference dye, because of its size. The Fast Green FCF might travel similarly to the orange reference dye. Betanin might migrate similarly to the red dye, and Carminic acid might travel similarly to the blue reference dye.
Dog manufacturers might put artificial food colors in their dog food because they want it to look more appealing to dog owners. It gives the food a more tasty appearance, making the owners want to purchase the food for their dog.
FD&C Dye
|
Color
|
Natural Alternative
|
Source of Natural Dye
|
Yellow 5
|
Yellow-Orange
|
Paprika
|
Paprika
|
Yellow 6
|
Yellow
|
Annatto
|
Achiote tree
|
Red 40
|
Red
|
Purple sweet potato
|
Purple sweet potato
|
Blue 1
|
Blue
|
Grape juice concentrate
|
Grapes
|
Red 3
|
Red
|
Beets
|
Beets
|
Two factors that controlled the distance that the colored dyes traveled is the size of the DNA fragments and the agarose gel that it's in. The force that helps move the dye through the gel is the electrical current. The DNA tends to move away from the negative side and to the positively charged side.
The gel causes the molecules to separate by size because it limits how far the fragments can travel through the gel. In the gel, there are little roadblocks that the fragments have to move around. The smaller the fragment, the faster and easier it can travel toward the positively charged side of the gel.
With molecules of 600, 1000, 2000, and 5000 daltons, I would expect the molecule with 600 daltons to move the furthest, because of its smallest size. The molecule with 5000 daltons would end up closest to the well where you put the molecules in the gel.
Comments
Post a Comment