In this lab, we asked the question, "Can macromolecules be identified in an egg cell?" We found that in an egg membrane there are polysaccharides, in egg whites there are proteins, and in an egg yolk there are lipids. When iodine is added to a substance and the substance turns black, it indicates that there are polysaccharides in it. The egg membrane mixture turned black when iodine was added to it. When sodium hydroxide and copper sulfate is added to a substance and it turns purple or light purple, it indicates that there are proteins present in that substance. The egg whites turned purple when we added sodium hydroxide and copper sulfate to it. When Sudan IV is added to a substance and it turns orange, it indicates that the substance contains lipids. The egg yolk turned orange after Sudan IV was added to it. This data supports our claim because the mixtures turned the specific colors that indicate that a certain macromolecule is in a mixture.
While our hypothesis supported our data, there could have been possible errors due to personal opinions on what color the mixtures turned and errors in measuring the solutions. Everyone's opinion differs when it comes to judgement of colors, so there could have been errors in the data due to what people thought the colors were and how much they thought the colors changed because everyone sees colors differently. Another reason for why there may have been errors in our experiment is there could have been problems measuring. When we needed 2 mL of the distilled water, many of us were confused on how to measure it since we didn't have any beakers of anything to put the water in first before we used the pipette to measure it. Many students tried measuring it by sticking the pipette into the stream of running water to measure it, which could have caused problems in measuring. There also could have been problems in measuring the egg. There wasn't really a way to get 2 mL of the egg membrane, and we were told to tear off a small piece to put in our test tubes. The egg yolk was also quite difficult to measure since it wouldn't cooperate with the pipette very well. Due to these errors, in future experiments I would recommend that we should all have a class average on what we think the color scale number would be so the data could be consistent. We should also have a beaker that we can all use to measure the distilled water with the pipette.
This lab was done to demonstrate our knowledge of the four macromolecules and locate where they are in a cell. From this lab I learned where the macromolecules are located in a cell as well as how to test for its presence. In class, we learned about the four big macromolecules as well as where they are located in a cell. Based on my experience from this lab, you could use this information to find out which parts of the egg is the healthiest for your diet or what to eat if you want to have protein. You could also use the information from this lab to know where each of the macromolecules are located in the cell.
While our hypothesis supported our data, there could have been possible errors due to personal opinions on what color the mixtures turned and errors in measuring the solutions. Everyone's opinion differs when it comes to judgement of colors, so there could have been errors in the data due to what people thought the colors were and how much they thought the colors changed because everyone sees colors differently. Another reason for why there may have been errors in our experiment is there could have been problems measuring. When we needed 2 mL of the distilled water, many of us were confused on how to measure it since we didn't have any beakers of anything to put the water in first before we used the pipette to measure it. Many students tried measuring it by sticking the pipette into the stream of running water to measure it, which could have caused problems in measuring. There also could have been problems in measuring the egg. There wasn't really a way to get 2 mL of the egg membrane, and we were told to tear off a small piece to put in our test tubes. The egg yolk was also quite difficult to measure since it wouldn't cooperate with the pipette very well. Due to these errors, in future experiments I would recommend that we should all have a class average on what we think the color scale number would be so the data could be consistent. We should also have a beaker that we can all use to measure the distilled water with the pipette.
This lab was done to demonstrate our knowledge of the four macromolecules and locate where they are in a cell. From this lab I learned where the macromolecules are located in a cell as well as how to test for its presence. In class, we learned about the four big macromolecules as well as where they are located in a cell. Based on my experience from this lab, you could use this information to find out which parts of the egg is the healthiest for your diet or what to eat if you want to have protein. You could also use the information from this lab to know where each of the macromolecules are located in the cell.
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| Each part of the egg is tested for polysaccharides. |
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