pGLO Lab Conclusion

Plate	Number of Colonies	Color of colonies under room light	Color of colonies under UV light	Comments
-pGLO LB	carpet	cloudy cream	cloudy cream	cover over whole plate
-pGLO LB/amp	-	-	-	no resilience
+pGLO LB/amp	~264	cream	cream	lots of small colonies
+pGLO LB/amp/ara	60	cream	fluorescent green	large colonies

What two new traits do your transformed bacteria have?

The colonies in the +pGLO/amp/ara glowed under UV light. They also became visible in all plates except

the -pGLO LB/amp.

Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.

Thousands of bacteria might have been in the 100uL of bacteria that we spread on each plate. There’s so many colonies in the +pGLO plates, and on the carpet, you can tell that there could have been

a lot of bacteria that created the carpet.

What is the role of arabinose in the plates?
Arabinose makes the bacteria glow under UV light.

List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.

1. GFP in animals- scientisis use GFP in animals and put them under UV light to see if it worked or not.
2. Jellyfish- jellyfish have GFP in their bodies which make them glow.
3. Fluorescence microscopy- scientisis use GFP in cells to find where tagged proteins are produced.

Give an example of another application of genetic engineering.

Cloning is an example of genetic engineering. It takes the DNA of one organism and inserts it into another organism that has no DNA so that it produces two organisms that are almost exactly alike.