To produce recombinant DNA (rDNA), you first need to isolate, then get a a restriction enzyme to cut the plasmid. Then you need to mix the plasmid with the insulin gene. Then you add ligase to attach the sticky ends and then mix the recombinant plasmid with the bacteria. 
To see if bacteria have taken in your plasmid, you could use tetracycline or ampicillin in your petri dish because the plasmid is not resistant to it. You couldn't use kanamycin because the plasmid is resistant to it.
Restriction enzymes are enzymes that cut DNA whenever it reads a specific sequence. We used Eco RI because it cut our plasmid in only one place and it cut our DNA in two places. If you used an enzyme that cut the plasmid in two places, the plasmid wouldn't be a ring, instead, it would be a long strip. This process is important in our daily life because it can result in GMOs and GMOs are used in 70% of processed foods because it enhances what is used in the processed foods. It can also help with genetic diseases in DNA. Recombinant DNA technology can be used in cloning organisms.
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